Rp-hplc Method for Simultataneous Determination of Irbesartan, Losartan, Hydro- Chlorothiazide and Chlorthalidone–application to Commercially Available Drug Products

Sharon Bio-Medicine Ltd, Plot L6, Taloja-MIDC, TalukaPanvel, Dist Raigad410 208, Maharashtra, India Shri Jagdishprasad Jhabarmal Tibrewala University, Vidya Nagari, Churu-Bishau Road, Chudela, Dist. Jhunjhunu333 001, Rajasthan, India ABSTRACT A simple, precise and stability-indicating HPLC method was developed and validated for the simultaneous determination of anti-hypertensive drugs Irbesartan, Losartan, diuretics Hydrochlorothiazide and Chlorthalidone. The separation was achieved on Hypersil BDS (Length 250 mm × Diameter 4.6 mm Particle size 5 μm) column with gradient flow. The mobile phase at a flow rate of 1.0 mL min consisted of 0.05 M sodium dihydrogen phosphate buffer and acetonitrile (Gradient ratio). The UV detection was carried out at 220 nm. The method was successfully validated in accordance to ICH guidelines. Further, the validated method was applied for commercially available pharmaceutical dosage form.